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A natural pressure gradient exists across cellular membranes, so that when a cell is placed in an electric eld, the transmembrane potential increases If the applied electrical eld is more than a certain critical value, then the cell wall ruptures While the complete mechanism of cell wall breakdown in an electric eld is not clearly understood, this observed phenomenon is used regularly in creating pores in a cell membrane The technique has been used in the eld of biotechnology to introduce foreign DNA into a cell A eld strength above 15 kV/cm is required for destruction of microbial membranes (6) In addition, reactive free radicals or electrolysis products are generated during PEF process that may also contribute to the microbial inactivation The use of intensive electric eld pulses on inactivation of microorganisms in foods was patented by Maxwell laboratories in the mid-1980s ( 2, 5). 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